Potassium influenced phenylalanine ammonia-lyase, peroxidases and polyphenol oxidases in Fusarium graminearum infected maize (Zea mays L.)
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Enzymes in Metabolism of Amino Acids. VII.* Phenylalanine Ammonia-Lyase in Maize {Zea mays L.)
We have isolated an enzyme, phenylalanine ammonia-lyase (EC 4.3.1.5.), from the rootlets of Zea mays var. СЕ-IV. After partial purification by fractional salting out with ammonium sulfate and gel chromatography on Sephadex G-100, we obtained a preparation of a 40-fold greater specific activity than that of the crude homogenate. We hbve determined the ap parent Michaelis constant and the activa...
متن کاملRoot Growth and Enzymes Related to the Lignification of Maize Seedlings Exposed to the Allelochemical L-DOPA
L-3,4-Dihydroxyphenylalanine (L-DOPA) is a known allelochemical exuded from the roots of velvet bean (Mucuna pruriens L. Fabaceae). In the current work, we analyzed the effects of L-DOPA on the growth, the activities of phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL), and peroxidase (POD), and the contents of phenylalanine, tyrosine, and lignin in maize (Zea mays) roots. Three-d...
متن کاملResponses of growth and antioxidative enzymes to various concentrations of nickel in Zea mays leaves and roots. Fatemeh Ghasemi*, Reza Heidari, Rashid Jameii and Latifeh Purakbar
To assess nickel-induced toxicity in plants, Zea mays seeds were germinated and cultured on nutrient solution with nickel concentrations of 50-200 μM for a period of two weeks. Observed biological makers included biomass, soluble and total protein contents, and the activities of guaiacol peroxidase (GPX), ascorbate peroxidase (APX), catalase (CAT), and phenylalanine ammonia-lyase (PAL) in the l...
متن کاملMembrane-bound guaiacol peroxidases from maize (Zea mays L.) roots are regulated by methyl jasmonate, salicylic acid, and pathogen elicitors
Plant peroxidases are involved in numerous cellular processes in plant development and stress responses. Four plasma membrane-bound peroxidases have been identified and characterized in maize (Zea mays L.) roots. In the present study, maize seedlings were treated with different stresses and signal compounds, and a functional analysis of these membrane-bound class III peroxidases (pmPOX1, pmPOX2...
متن کاملl-Phenylalanine Ammonia-lyase (Maize): Partial Purification and Response to Gibberellic Acid and Cycloheximide of l-Phenylalanine and l-Tyrosine Ammonia-lyase Activities.
Extracts of maize leaf sheath tissue deaminate both l-phenylalanine and l-tyrosine. The activities with both substrates are enhanced by treating the plant with gibberellic acid. Both activities decrease rapidly at the same rate when tissue is incubated in a moist atmosphere, and this decrease can be slowed by treatment with cycloheximide. The ratio of the activities was constant throughout a se...
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تاریخ انتشار 2009